OBJECTIVES: To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals. RESULTS: By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnR c/araR c was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains. CONCLUSIONS: Mixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency.
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机译:目的:提高酶促水解的效率,以将植物生物质转化为可再生生物燃料和化学品。结果:通过在里氏木霉中过表达点突变A824 V转录激活因子Xyr1,最佳突变体的羧甲基纤维素酶,纤维二糖苷酶和β-D-葡萄糖苷酶活性从1.8 IU / ml,0.1 IU / ml和0.05 IU / ml增加到4.8 IU / ml,0.4 IU / ml和0.3 IU / ml。通过组合来自该突变体和黑曲霉基因修饰菌株ΔcreA/ xlnR c / araR c的酶,小麦秸秆糖化的糖产量提高至7.5 mg / ml,与野生型菌株的组合相比提高了229%。结论:来自里氏木霉和黑曲霉的混合酶与转录因子的遗传修饰相结合是提高糖化效率的有前途的策略。
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